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Procedures

Cambridge Resource Centre for Comparative Genomics

Procedure

Requests for chromosome specific DNA should be sent to the Resource Centre and be accompanied by a project outline so that the Centre can determine the level of work required (see Application check list with the Agreement).

It should be noted:-

  1. Chromosome-specific DNA will be supplied only for non-commercial purposes.
  2. The originator of the request will normally be responsible for providing a clean, actively growing cell line suitable for preparing chromosome suspensions for sorting.
  3. The material supplied will consist of unlabelled secondary PCR amplification products of sorted chromosomes, together with a flow karyotype indicating the peak of origin of the sorted chromosomes.
  4. Normally, the investigator making the request will be responsible for identifying the chromosome of origin of the amplified DNA by labelling and hybridisation to metaphase preparations in his/her own laboratory.
  5. If (4) is not possible, arrangements may be made for the investigator to undertake chromosome verification at the Centre with the help of the staff of the Centre. Arrangements for visiting the Centre must be made at the investigator's expense.
  6. Requests for undertaking collaborative work with the Centre can be considered.

Additional Considerations

  1. Confidentiality. All applications are treated in strict confidence.
  2. Cell lines will be stored in the Centre's cell bank at the owner's risk. No guarantee can be given that stored lines can be successfully recovered, although every effort will be made to ensure the safety of each deposit.
  3. Chromosome-specific DNA from deposited cell lines will be prepared for other investigators only with the written consent of the depositor. Where other projects are not in conflict with the original project, permission for preparing chromosome-specific DNA should not normally be refused, provided that an assurance can be given not to pass on the material to other laboratories without the depositor's permission.
  4. The Centre retains the right to store chromosome-specific DNA from each flow-sorting experiment it undertakes as such material could be of value to the investigator or to other investigators (with permission) in the future.
  5. Stored chromosome-specific DNA will be available for distribution for research purposes to members of the comparative genomics community on request, after the lapse of two years, or at such time as agreed with the depositor.

Agreement

You are required to print out, sign and post an Agreement with Resource Centre for Comparative Genomics if you make an application for resources.