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Department of Veterinary Medicine

Cambridge Veterinary School

Studying at Cambridge

 

Dr John Gibson

Dr John Gibson

Reader in Pathophysiology

Ion and water homeostasis in cells

Red blood cell pathophysiology

Sickle cell disease

John Gibson is interested in taking PhD students.


Office Phone: +44 (0)1223 337638

Subject groups/Research projects

Comparative Pathobiology:

Research Interests

Our laboratory is interested in how cells carry out ion and water homeostasis, and how this homeostasis may be perturbed in disease. In particular, we examine the regulation and activity of key membrane transporters in cellular pathophysiology. We are mainly concerned with the red cell,and also articular chondrocytes. Projects include altered ion balance in sickle cell disease (the commonest severe inherited disorder in people) and also other haemoglobinopathies, together with the effects of modulators such as oxidants. Oxygen tension has particularly important consequences on ionic balance in red cells and represents an important focus of our work. Latterly, work has also concerned how altered transport affects exposure of phosphatidylserine on the surface of red cells, and thereby alters their ability to traverse the microvasculature. In addition, we have successfully applied electrophysiological techniques to record whole-cell conductance and single channel activity from sickle red cells. We also examine oxidative stress in red blood cells.  Our aim is to understand altered permeability in sickle cells, to correct it and thereby ameliorate some of the complications of the disease.  Funding sources include Medical Research Council, Wellcome Trust, British Heart Foundation, BBSRC and Action Medical Research.

Main collaborators

  • Prof Clive Ellory, University of Oxford
  • Dr David Rees, King's College Hospital London
  • Dr Yu-Ling Ma, University of Oxford
  • Dr Robert Wilkins, University of Oxford
  • Prof Clinton Joiner, Cincinnati Comprehensive Sickle Cell Center
  • Prof Pat Gallagher, Yale Medical School
  • Prof Andy Cossins, University of Liverpool

Keywords

  • cellular pathophysiology
  • articular chondrocytes
  • homeostasis

Key Publications

  • Hannemann, A., Cytlak, U. M., Gbotosho, O. T., Rees, D. C., Tewari, S. & Gibson, J. S. (2013).  Effects of o-vanillin on K+ transport of red blood cells from patients with sickle cell disease.  Blood Cells, Molecules & Disease In press.

  • Cytlak, U. M., Hannemann, A., Rees, D. C. & Gibson, J. S. (2013).  Identification of the Ca2+ entry pathway involved in deoxygenation-induced phosphatidylserine exposure in red blood cells from patients with sickle cell disease.  Pflug Archiv.  465, 2652-2660.

  • Milligan, C., Rees, D. C., Ellory, J. C., Osei, A., Browning, J. A., Hannemann, A. & Gibson, J. S. (2013).  A non-electrolyte haemolysis assay for diagnosis and prognosis of sickle cell disease.  J. Physiol. 591, 1463-1474.

  • Milner, P. I., Smith, H. C., Robinson, R., Wilkins, R. J. & Gibson, J. S. (2013).  Growth factor regulation of intracellular pH homeostasis under hypoxic conditions in isolated equine articular chondrocytes.  J. Orthop. Res.  31, 197-203.
  • Ma, Y.-L., Rees, D. C., Gibson, J. S. & Ellory, J. C. (2012). The conductance of red blood cells from sickle cell patients: ion selectivity and inhibitors. J. Physiol. 590, 2095-2105.[PubMed].
  • Rees, D. C. & Gibson, J. S. (2012). Biomarkers in sickle cell disease. Br. J. Haematol. 156, 433-445.[PubMed].
  • Weiss, E., Cytlak, U., Rees, D. C., Osei, A. & Gibson, J. S. (2012). Deoxygenation-induced and Ca2+dependent phosphatidylserine externalisation in red blood cells from normal individuals and sickle cell patients. Cell Calcium 51, 51-56.[PubMed].
  • Hannemann, A., Weiss, E., Rees, D. C., Dalibalta, S., Ellory, J. C. & Gibson, J. S. (2011). The properties of red blood cells from patients heterozygous for HbS and HbC (HbSC genotype). Anemia 2011, 248527, 1-8.[PubMed].
  • White, R. & Gibson, J. S. (2010). The effect of oxygen tension on calcium homeostasis in bovine articular chondrocytes. J. Ortho. Res. Surg. 5, 27-32.[PubMed].
  • Dalibalta, S., Ellory, J. C., Browning, J. A., Wilkins, R. J., Rees, D. C. & Gibson, J. S. (2010). Novel permeability characteristics of red blood cells from sickle cell patients heterozygous for HbS and HbC (HbSC genotype). Blood Cells Mol. Dis. 45, 46-52.[PubMed].
  • Gibson, J. S., McCartney, D., Sumpter, J., Fairfax, T. P. A., Milner, P. I., Edwards, H. L. & Wilkins, R. J. (2009). Rapid effects of hypoxia on H+ homeostasis in articular chondrocytes. Pflug. Arch. 458, 1085-1092.[PubMed].
  • Gibson, J. S., Milner, P. I., White, R., Fairfax, T. P. A. & Wilkins, R. J. (2007). Oxygen and reactive oxygen species in articular cartilage: modulation of ionic homeostasis. Pflugers Archiv In press.[PubMed].
  • Browning, J. A., Staines, H. M., Robinson, H. C., Powell, T., Ellory, J. C. & Gibson, J. S. (2007). The effect of deoxygenation on whole-cell conductance of red blood cells from normal individuals and sickle cell patients. Blood 109, 2622-2629.[PubMed].
  • Browning, J. A., Robinson, H. C., Ellory, J. C. & Gibson, J. S. (2007). Deoxygenation-induced non-electrolyte pathway in red cells from sickle cell patients. Cell. Physiol. Biochem. 19, 165-174.[PubMed].
  • Milner, P. I., Fairfax, T. P. A., Browning, J. A., Wilkins, R. J. & Gibson, J. S. (2006). The effect of oxygen tension on pH homeostasis in equine articular chondrocytes. Arthritis Rheum. 54, 3523-3532.[PubMed].