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Department of Veterinary Medicine

Cambridge Veterinary School

Studying at Cambridge

 

Dr Sebastian Bruchmann

Dr Sebastian Bruchmann

Research Associate


Biography:

I am a postdoctoral fellow in Julian Parkhill’s group with an interest in microbial functional genomics, in particular Klebsiella pneumoniae) and other Enterobacteriaceae species (Salmonella Typhimurium, Enterobacter cloacae).

After graduating with a BSc in Biology and a MSc in Biology with majors in Microbiology and Genetics from the Technical University of Braunschweig/Germany, I joined to lab of Susanne Häussler at the Helmholtz Centre for Infection Research. During my PhD I used deep transcriptomic sequencing (RNA-seq) to study Antimicrobial Resistance in Pseudomonas aeruginosa and Klebsiella pneumoniae. Following a short post-doc in the same group where I developed rapid molecular diagnostics to detect hospital outbreaks and antibiotic resistance in several different bacterial species (mainly, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and Clostridium difficile).

In 2017, I took up a post as a postdoctoral fellow in the Parkhill group at the Wellcome Sanger Institute using Functional Genomics (RNA-sequencing and transposon directed sequencing, TraDIS) to understand the Antimicrobial stress response in the Enterobacteriaceae species Klebsiella, Salmonella and Enterobacter. My work at the Department of Veterinary Medicine will focus on the Interaction of Klebsiella pneumoniae with the immune system, in particular macrophages.

Subject groups/Research projects

Infection and Immunity:
Mathematical Biology:

Research Interests

My main research interests are antimicrobial resistance in Enterobacteriaceae and host-pathogen interactions of Klebsiella pneumoniae. I am using different sequencing-based methods to understand how the bacterium copes with certain stresses and which genes are important during different conditions. I want to know how, when and why genes and their products are used.

Wikipedia describes “Functional Genomics” as “[..] a field of molecular biology that attempts to describe gene (and protein) functions and interactions. Functional genomics make use of the vast data generated by genomic and transcriptomic projects (such as genome sequencing projects and RNA sequencing) [and] focuses on the dynamic aspects such as gene transcription, translation, regulation of gene expression and protein–protein interaction […]. A key characteristic of functional genomics studies is their genome-wide approach to these questions, generally involving high-throughput methods rather than a more traditional “gene-by-gene” approach.”

 

Keywords

  • TraDIS
  • Klebsiella pneumoniae
  • Functional Genomics
  • Host-Pathogen Interaction
  • Antimicrobial resistance
  • Microbiology
  • RNA-seq
  • CRISPR-Cas9

Key Publications

Bense S, Bruchmann S, Steffen A, Stradal TEB, Häussler S, Düvel J. Spatiotemporal control of FlgZ activity impacts Pseudomonas aeruginosa flagellar motility. Mol Microbiol. 2019

Lorenz A, Preuße M, Bruchmann S, Pawar V, Grahl N, Pils MC, Nolan LM, Filloux A, Weiss S, Häussler S. Importance of flagella in acute and chronic Pseudomonas aeruginosa infections. Environ Microbiol. 2019

Khaledi A, Schniederjans M, Pohl S, Rainer R, Bodenhofer U, Xia B, Klawonn F, Bruchmann S, Preusse M, Eckweiler D, Dötsch A, Häussler S. Transcriptome Profiling of Antimicrobial Resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2016

Bruchmann S, Muthukumarasamy U, Pohl S, Preusse M, Bielecka A, Nicolai T, Hamann I, Hillert R, Kola A, Gastmeier P, Eckweiler D, Häussler S. Deep transcriptome profiling of clinical Klebsiella pneumoniae isolates reveals strain and sequence type-specific adaptation. Environ Microbiol. 2015

Bruchmann S, Dötsch A, Nouri B, Chaberny IF, Häussler S. Quantitative contributions of target alteration and decreased drug accumulation to Pseudomonas aeruginosa fluoroquinolone resistance. Antimicrob Agents Chemother. 2013